Novo badnavírus em infecção mista com begomovírus em Centrosema brasilianum

ABSTRACT: Plant viruses have a wide range of weed/wild hosts, which can act as virus reservoirs. Centosema brasilianum is wild leguminous species frequently found in association with cultivated fields the Northeast region country. In March 2017, plant C. was showing symptoms mosaic and leaf rolling, municipality Maceio, state Alagoas. this context, present study aimed to detect molecular characterize DNA genomes infecting brasilianum. Total extracted from sample used template via PCR amplification reactions assays using universal primers, aiming respectively at detection badnavirus begomovirus. Partial sequences were obtained for RT/RNaseH begomovirus DNA-A regions. Comparisons paired phylogenetic analyzes indicated presence coinfection by The isolate BR:Mac:17 should be considered putative new badnavirus, name ‘Centrosema bacilliform virus’ (CenBV) proposed. This phylogenetically more related Dioscorea AL (DBALV, KX008573), rotundata. Meanwhile, BR:Mac2:17 Begomovirus, probably Bean golden (BGMV). results demonstrate that reservoir absence culture field. KEYWORDS: Molecular characterization, Caulimoviridae, Geminiviridae, non-cultivated plants.